A simple, economical, accurate, and, and reproducible spectrophotometric method for the simultaneous estimation of Nebivolol and Hydrochlorothiazide in combined dosage forms. The method was based on dual wavelength data processing program (dual wavelength Spectrophotometry or DW Spectrophotometry). In the proposed method, Absorbance spectrum of pure NEB was scanned in the spectrum basic mode. NEB showed some absorbance value at 286.0 nm while it does not show any absorbance value at 315.5 nm. The absorbance value at 315.5 nm is due to HTZ only in the combined mixture of both drugs. Wavelength 315.5 nm was selected for the measurement of HTZ and 286.0 nm for NEB. Linearity range was observed in the concentration range of 4-24 μg/ml for Nebivolol and 10-60 μg/ml for Hydrochlorothiazide. Developed method was successfully applied for simultaneous determination of NEB and HTZ in combined dosage forms that are available in the market. The method was validated statistically and recovery study was performed to confirm the accuracy of the method. The method was found to be rapid, simple, accurate and precise.
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